BM 2205 BIOCHEMISTRY L T P C
3 0 0 3
AIM
> To study the biochemical reactions and the various methods to analyze them.
OBJECTIVE:
> To give a clear understanding of important biomolecules and their functions. > To analyze the metabolic pathways in normal and diseased state.
> To help in devising analytical & diagnostic tools.
UNIT I 6
Introduction to biochemistry – Biomolecules, structure of water & its importance – Important noncovalent forces – Hydrogen bonds, electrostatic, hydrophobic & vanderwaals forces – Acid, base & buffers – pH, Henderson Hasselbalch equation. Biological buffers and their significance – Principle of viscosity – surface tension , adsorption, diffusion, osmosis & their applications in biological systems.
UNIT II 9
Classification, structure & properties of carbohydrates – mono, di , oligo & olysaccharides. Classification, structure & properties of amino acids & proteins.
Classification, structure & properties of Lipids – Simple lipids , Phospholipids , glycolipids & steroids . Transport of lipids: Lipoproteins Structure & functions of nucleic acids – Nucleosides , nucleotides – Cyclic AMP , cyclic GMP , ATP , GTP – DNA & RNA
UNIT III 12
Classification of Enzymes, Chemical nature, Active Site, Specificity of Enzyme catalyzed reactions, Regulation : Feedback , Allosteric , Covalent modification , Hormonal regulation, co-enzymes. Assay of enzymes, enzymes in clinical diagnosis of diseases. Introduction to Metabolism: Carbohydrate metabolism, Glycolysis
Lipid metabolism : fatty acid, beta oxidation , ketogenesis and cholesterol metabolism. TCA cycle : Structure of biological membranes, electron transport & Oxidative phosphorylation.
UNIT IV 9
Liver function and liver function tests, Kidney function and kidney function tests , normal & abnormal constituents of urine and their clinical significance. General characteristics of hormones. Structure , functions & disorders of thyroid , parathyroid , pituitary ,adrenal and pancreatic hormones. Hormones as chemical messengers: General assay of hormones – Bio assay , chemical assay & immuno assays.
UNIT V 9
Analytical techniques: Principle and applications of electrophoresis – PAGE , SDS PAGE , Isoelectric focusing , Two Dimensional Electrophoresis. Chromatography: Principle of adsorption & partition chromatography, Size exclusion , Ion exchange & affinity chromatography. Spectro photometry, fluorimetry, flame photometry, manometry , microcalorimetry , electrochemical methods, biosensors , automation in clinical laboratory , use of radio isotopes in biochemistry.
TOTAL : 45 PERIODS
TEXT BOOKS:
1. Harper’s review of biochemistry By David. W. Martin, Peter. A. Mayes , Victor. W. Rodwell . LANGE medical publications, 2003.
2. Practical Biochemistry – Principles & Techniques,Keith Wilson & John Walker. Oxford university press.
EFERENCE S:
1. Understanding Enzymes By Trevor palmer. Published by Ellis Horwood LTD.
2. Biochemistry Lippincott’s Illustrated Reviews By Pamela.C.Champe & Richard. A.Harvey. Lippincott-Raven publishers, 3rd edition, 2006.
3 0 0 3
AIM
> To study the biochemical reactions and the various methods to analyze them.
OBJECTIVE:
> To give a clear understanding of important biomolecules and their functions. > To analyze the metabolic pathways in normal and diseased state.
> To help in devising analytical & diagnostic tools.
UNIT I 6
Introduction to biochemistry – Biomolecules, structure of water & its importance – Important noncovalent forces – Hydrogen bonds, electrostatic, hydrophobic & vanderwaals forces – Acid, base & buffers – pH, Henderson Hasselbalch equation. Biological buffers and their significance – Principle of viscosity – surface tension , adsorption, diffusion, osmosis & their applications in biological systems.
UNIT II 9
Classification, structure & properties of carbohydrates – mono, di , oligo & olysaccharides. Classification, structure & properties of amino acids & proteins.
Classification, structure & properties of Lipids – Simple lipids , Phospholipids , glycolipids & steroids . Transport of lipids: Lipoproteins Structure & functions of nucleic acids – Nucleosides , nucleotides – Cyclic AMP , cyclic GMP , ATP , GTP – DNA & RNA
UNIT III 12
Classification of Enzymes, Chemical nature, Active Site, Specificity of Enzyme catalyzed reactions, Regulation : Feedback , Allosteric , Covalent modification , Hormonal regulation, co-enzymes. Assay of enzymes, enzymes in clinical diagnosis of diseases. Introduction to Metabolism: Carbohydrate metabolism, Glycolysis
Lipid metabolism : fatty acid, beta oxidation , ketogenesis and cholesterol metabolism. TCA cycle : Structure of biological membranes, electron transport & Oxidative phosphorylation.
UNIT IV 9
Liver function and liver function tests, Kidney function and kidney function tests , normal & abnormal constituents of urine and their clinical significance. General characteristics of hormones. Structure , functions & disorders of thyroid , parathyroid , pituitary ,adrenal and pancreatic hormones. Hormones as chemical messengers: General assay of hormones – Bio assay , chemical assay & immuno assays.
UNIT V 9
Analytical techniques: Principle and applications of electrophoresis – PAGE , SDS PAGE , Isoelectric focusing , Two Dimensional Electrophoresis. Chromatography: Principle of adsorption & partition chromatography, Size exclusion , Ion exchange & affinity chromatography. Spectro photometry, fluorimetry, flame photometry, manometry , microcalorimetry , electrochemical methods, biosensors , automation in clinical laboratory , use of radio isotopes in biochemistry.
TOTAL : 45 PERIODS
TEXT BOOKS:
1. Harper’s review of biochemistry By David. W. Martin, Peter. A. Mayes , Victor. W. Rodwell . LANGE medical publications, 2003.
2. Practical Biochemistry – Principles & Techniques,Keith Wilson & John Walker. Oxford university press.
EFERENCE S:
1. Understanding Enzymes By Trevor palmer. Published by Ellis Horwood LTD.
2. Biochemistry Lippincott’s Illustrated Reviews By Pamela.C.Champe & Richard. A.Harvey. Lippincott-Raven publishers, 3rd edition, 2006.
No comments:
Post a Comment